Innovative Probiotic Lactic Yeast for the pharmaceutical, nutritional and agricultural industries

Trial#27: Utilization of probiotics with a basis of typified lactic yeasts (Turval B0399) in the diet of sleddogs (huskies).

Ararad Khatchikian – Huskies breeder and teacher of the Mushing international school of Tarvisio (Italy)

Object: trial No. 27
Trial of utilization of probiotics with a basis of typified lactic yeasts (Turval B0399) in the diet of sleddogs (huskies).
Ararad Khatchikian – Huskies breeder and teacher of the Mushing international school of Tarvisio (Italy)
Objective.
The objective of the trial was to observe the effects of the utilization of the new probiotics with a basis of typified lactic yeasts Kluyveromyces B0399 (Turval B0399) in the diet of sleddogs.
In particular, the digestive functions and general health of the dogs were observed.
 
Summary of results.
Individual charts were kept showing results relative to 7 dogs (3 male and 4 female) of ages between 13 months and 10 years.
Except for the dog Mille (charts 7 and 8), whose results were not evident due to a severe case of verminosis, the other 6 dogs had positive results with the addition of the probiotic Turval B0399; especially Alfa (charts 1 and 2), Yerghu (charts 9 and 10) and Yeregh (charts 11 and 12).
The product demonstrated to be most certainly valid and very interesting.
 
Materials

a- Base diet: Mixed: Croquettes + warm water (300 g + 2 liters of

water in 2 meals a day.)

b- probiotic integration: TURVAL 25 DOG
Ingredients:Turval 25 dog is comprised of a balanced mixture of natural products: 80% TURVAL B0399 (typified active lactic yeast Kluyveromyces F. B0399, wheat bran pre-treated and sterilized, whey powder, lactose, hydrolysed casein ), 20% fine bran of wheat.
Manufacturing company: TURVAL Laboratories, Ltd. Pradamano, Udine, Italy. Authorisation Min. Health No. IT M 00001 UD.
 
Place of testing:
SCUOLA INTERNAZIONALE MUSHING Via Rutte Grande, 16 - C.P. 13633018 TARVISIO (UD) Italy Fax +39 0428 40566
 
Methods and results
See attached charts 


 
CHART 1
GENERAL INFORMATION                                                                               Subject treated: ALFA
 
Breed: HUSKY
Sex: FEMALE
Age: YEARLING - 13 MONTHS OLD
State of health at beginning of trial:

(Critical) Convalescence due to both a heavy hormonal treatment (which provoked a

vaginal infection) and antibiotic treatment. The dog was extremely weak and was

being administered phleboclyses of hysiological solution.

Duration of treatment: 4 weeks
Dosage used: 12 g in the morning meal
Incoveniences encountered during period of administration : none
 
CHART 2 

DIARY CHART                                                                       

Subject treated: ALFA

 
Observations during the course of administration period
General state of health
Beginning of administration
 
1st week
 
2nd week
 
3rd week
 
4th week
 
5th week
Excellent
 
 
 
 
 
 
Good
 
 
 
X
X
 
Fair
 
 
X
 
 
 
Critical
X
X
 
 
 
 
Feces: consistency
Liquid
X
 
 
 
 
 
Soft
 
X
 
 
 
 
Normal
 
 
X
X
X
 
Dry
 
 
 
 
 
 
 
Feces: odor
More acrid than normal
X
 
 
 
 
 
Normal
 
 
X
X
X
 
Less acrid than normal
 
X
 
 
 
 
 
 
 
 
 
 
 
Coat
Shiny
 
 
X
X
X
 
Dull
X
X
 
 
 
 
Crinkled
 
 
 
 
 
 
 
 
 
 
 
 
 
Eyes
Lively
 
 
 
X
X
 
Normale
 
X
X
 
 
 
Dull
X
 
 
 
 
 
 
 
 
 
 
 
 
 
Weight of subject
 
15 kg
 
16 kg
 
17 kg
 
19 kg
 
20 kg
 
 
 
Final comment :
At the beginning of the trial with TURVAL B0399 the dog was very weak and underwent fleboclyses of physiological solution. TURVAL B0399 definitely contributed in awakening its appetite and rebuilding the bacteric flora


 
CHART 3 

GENERAL INFORMATION                                                                  

Subject treated: ELIM

 
Breed: HUSKY
Sex: FEMALE
Age: ADULT - 7 YEARS OLD
State of health at beginning of trial: (fair) At the beginning of administration the dog had an intestinal parassitosis brought on by whipworm, which did not intefere with appetite and general behavior.
Duration of treatment: 4 weeks
Dosage used: 12 g in the morning meal
Inconveniences encountered during period of administration: none

 

 

CHART 4

DIARY CHART                                                                                   

Subject treated: ELIM

 
Observations during the course of administration period
General state of health
Beginning of administration
 
1st week
 
2nd week
 
3rd week
 
4th week
 
5th week
Excellent
 
 
 
 
 
 
Good
 
X
X
X
X
 
Fair
X
 
 
 
 
 
Critical
 
 
 
 
 
 
 
·       Feces: consistency
Liquid
X
 
 
 
 
 
Soft
 
X
X
X
X
 
Normal
 
 
 
 
 
 
Dry
 
 
 
 
 
 
 
Feces: odor
More acrid than normal
X
 
 
 
 
 
Normal
 
 
 
 
 
 
Less acrid than normal
 
X
X
X
X
 
 
 
 
 
 
 
 
·       Coat
Shiny
 
X
X
X
X
 
Dull
X
 
 
 
 
 
Crinkled
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Eyes
Lively
 
 
 
 
 
 
Normal
X
X
X
X
X
 
Dull
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Weight of subject
 
20 kg
 
20 kg
 
21 kg
 
21 kg
 
22 kg
 
 
 
 
 
 
 
 
 
 
Final comment:
TURVAL B0399 improved the consistency of the feces though it did not resolve the problem of verminosis. After the fifth week the dog was dewormed and the feces returned to normal.


CHART 5

GENERAL INFORMATION                                                                  

Subject treated: SCREAMER

 
Breed: HUSKY
Sex: FEMALE
Age: YEARLING - 20 MONTHS
Duration of treatment: 4 weeks
Dosage used:12 g in the morning meal
State of health at beginning of trial: (good) At the beginning of administration the dog was in heat and therefore had a lack of appetite.
Inconveniences encountered during period of administration: none
 
CHART 6

DIARY CHART                                                                                   

Subject treated: SCREAMER

 
Observations during the course of administration period
General state of health
Beginning of administration
 
1st week
 
2nd week
 
3rd week
 
4th week
 
5th week
Excellent
 
 
 
 
 
 
Good
X
X
X
X
X
 
Fair
 
 
 
 
 
 
Critical
 
 
 
 
 
 
 
·       Feces: consistency
Liquid
 
 
 
 
 
 
Soft
 
 
 
 
 
 
Normal
X
X
X
X
X
 
Dry
 
 
 
 
 
 
 
Feces: odor
More acrid than normal
 
 
 
 
 
 
Normal
X
X
X
X
X
 
Less acrid than normal
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Coat
Shiny
X
X
X
X
X
 
Dull
 
 
 
 
 
 
Crinkled
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Eyes
Lively
 
 
 
 
 
 
Normal
X
X
X
X
X
 
Dull
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Weight of subject
 
17 kg
 
17 kg
 
17 kg
 
18 kg
 
18 kg
 
 
 
 
 
 
 
 
 
 
Final comment:
TURVAL B0399 was administered to stimulate the poor appetite caused by the dog’s being in heat. The dog found it extremely palatable and even loved eating it in the bowl with no other additions.


 
CHART 6
GENERAL INFORMATION                                                                   
Subject treated: MILLIE 
 
Breed: HUSKY
Sex: FEMALE
Age: ADULT - 5 YEARS AND 11 MONTHS OLD
State of health at beginning of trial : (fair) At the beginning of administration the dog had no appetite, weakened by an intestinal parassitosis caused by whipworm.
Duration of treatment: 4 weeks
Dosage used : 12 g in the morning meal
In conveniences encountered during period of administration: none
 
CHART 7

DIARY CHART                                                                                      

Subject treated: MILLIE

 
Observations during the course of administration period
General state of health
Beginning of administration
 
1st week
 
2nd week
 
3rd week
 
4th week
 
5th week
Excellent
 
 
 
 
 
 
Good
 
 
 
 
 
 
Fair
X
X
X
X
X
 
Critical
 
 
 
 
 
 
 
·       Feces: consistency
Liquid
 
 
 
 
 
 
Soft
X
X
X
X
X
 
Normal
 
 
 
 
 
 
Dry
 
 
 
 
 
 
 
Feces: odor
More acrid than normal
X
X
X
X
X
 
Normal
 
 
 
 
 
 
Less acrid than normale
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Coat
Shiny
 
 
 
 
 
 
Dull
X
X
X
X
X
 
Crinkled
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Eyes
Lively
 
 
 
 
 
 
Normal
X
X
X
X
X
 
Dull
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Weight of subject
 
20 kg
 
20 kg
 
20 kg
 
20 kg
 
20 kg
 
 
 
 
 
 
 
 
 
Final comment :
The probiotic did not give results in this subject . The successive deworming after treatment with TURVAL solved the problem completely.


 
CHART 8
GENERAL INFORMATION                                                                             Treated subject:  YERGHU’
 
Breed: HUSKY
Sex: MALE
Età:ADULTO - 6 YEARS OLD
State of health at beginning of trial : (excellent) : The dog’s feces have always had a tendency of being soft and fetid
Duration of treatment : 4 weeks
Dosage used : 12 g in the morning meal
Inconveniences encountered during administration: none
 
CHART 9

DIARY CHART                                                                                    

Treated subject: YERGHU’

 
Observations during the course of administration period
General state of health
Beginning of administration
 
1st week
 
2nd week
 
3rd week
 
4th week
 
5th week
Excellent
X
X
X
X
X
 
Good
 
 
 
 
 
 
Fair
 
 
 
 
 
 
Critical
 
 
 
 
 
 
 
·       Feces: consistency
Liquid
 
 
 
 
 
 
Soft
X
 
 
 
 
 
Normal
 
X
X
X
X
 
Dry
 
 
 
 
 
 
 
Feces: odor
More acrid than normal
X
 
 
 
 
 
Normal
 
X
X
X
X
 
Less acrid than normal
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Coat
Shiny
 
X
X
X
X
 
Dull
X
 
 
 
 
 
Crinkled
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Eyes
Lively
 
 
 
 
 
 
Normal
X
X
X
X
X
 
Dull
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Weight of subject
 
22 kg
 
22 kg
 
23 kg
 
23 kg
 
24 kg
 
 
 
 
 
 
 
 
 
Final comment :
TURVALB0399 definitely changed the consistency and odor of the feces, contributing in improving the general state of health of the dog. The supplement proved to be quite palatable, even on its own.


CHART 10
GENERAL INFORMATION                                                                               Subject treated: YERèGH
 
Breed: HUSKY
Sex:     MALE
Age: ADULT - 6 YEARS OLD
State of health at beginning of trial: (excellent) The dog has always had a tendency towards soft and fetid feces
Duration of the treatment: 4 weeks
Dosage used :12 g in the morning meal
Inconveniences encountered during period of administration: none
 
CHART 11 

DIARY CHART                                                                       

Subject treated: YERèGH

 
Observations during the course of administration period
General state of health
Beginning of administration
 
1st week
 
2nd week
 
3rd week
 
4th week
 
 
5th week
Excellent
X
X
X
X
X
 
Good
 
 
 
 
 
 
Fair
 
 
 
 
 
 
Critical
 
 
 
 
 
 
 
·       Feces: consistency
Liquid
 
 
 
 
 
 
Soft
X
 
 
 
 
 
Normal
 
X
X
X
X
 
Dry
 
 
 
 
 
 
 
Feces: odor
More acrid than normal
X
 
 
 
 
 
Normal
 
X
X
X
X
 
Less acrid than normal
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Coat
Shiny
 
X
X
X
X
 
Dull
X
 
 
 
 
 
Crinkled
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Eyes
Lively
 
 
 
 
 
 
Normal
X
X
X
X
X
 
Dull
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Weight of subject
 
22 kg
 
22 kg
 
23 kg
 
23 kg
 
24 kg
 
 
 
 
 
 
 
 
 
 
Final comment:
TURVAL B0399 definitely changed the consistency and odor of the feces, contributing in improving the general state of health of the dog. The supplement was extremelyl palatable and was also enjoyed on its own without other additions
 
CHART 12
GENERAL INFORMATION                                                                               Subject treated: SIMON
Breed:             HUSKY
Sex: MALE
Age: ADULT - 10 YEARS AND SIX MONTHS OLD
State of health at beginning of trial: (good) At the beginning of administration the dog had a parassitosis caused by whipworm which did not interfere with appetite or general behavior.
Duration of treatment: 4 weeks
Dosage used: 12 g in the morning meal
Inconveniences encountered during administration : none
 
 
CHART 13

Diary Chart                                                                                       

Subject treated: SIMON

 
Observations during the course of administration
General state of health
Beginning of administration
 
1st week
 
2nd week
 
3rd week
 
4th week
 
5th week
Excellent
 
 
 
 
 
 
Good
X
X
X
X
X
 
Fair
 
 
 
 
 
 
Critical
 
 
 
 
 
 
 
·       Feces: consistency
Liquid
X
X
X
 
 
 
Soft
 
 
 
X
X
 
Normal
 
 
 
 
 
 
Dry
 
 
 
 
 
 
 
Feces: odor
More acrid than normal
X
X
X
 
 
 
Normal
 
 
 
 
 
 
Less acrid than normal
 
 
 
X
X
 
 
 
 
 
 
 
 
·       Coat
Shiny
X
X
X
X
X
 
Dull
 
 
 
 
 
 
Crinkled
 
 
 
 
 
 
 
 
 
 
 
 
 
·       Eyes
Livlier
 
 
 
 
 
 
Normal
X
X
X
X
X
 
Dull
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Weight of subject
 
26 kg
 
26 kg
 
26 kg
 
26 kg
 
26 kg
 
 
 
 
 
 
 
 
 
 
Final comment:
TURVAL B0399 improved the consistency of the feces though it did not resolve the problem of verminosis. After the fifth week the dog was dewormed and the feces returned to normal.
 

Laboratori Turval Italia Srl – Head Office and Research Dpt: Scientific and Technological Park "L. Danieli"
via Jacopo Linussio, 51 – 33100 Udine (UD) – P. IVA 01883020305